14.5 Folate (categories 2)

Folate range (<40 vs >=40)

14.5.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 229.3346032 1 <0.0001 ***
Folate_gp2 2.4595598 1 0.1 ns
Study 0.5016894 1 0.5 ns
GA_samp 173.6025363 1 <0.0001 ***
Folate_gp2:Study 1.6178809 1 0.2 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 41.6985648 1 <0.0001 ***
Folate_gp2 0.3767370 1 0.5 ns
Study 13.4402945 1 0.0002 ***
GA_samp 2493.7981827 1 <0.0001 ***
Folate_gp2:Study 0.1843189 1 0.7 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 416.3034217 1 <0.0001 ***
Folate_gp2 0.5199859 1 0.5 ns
Study 0.0000572 1 1.0 ns
GA_samp 1063.6192459 1 <0.0001 ***
Folate_gp2:Study 0.2606543 1 0.6 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp2 1 Inf 0.141 0.141 0.7 ns
3 Study 1 Inf 10.517 10.517 0.001 **
4 GA_samp 1 Inf 0.967 0.967 0.3 ns
2 Folate_gp2:Study 1 Inf 3.702 3.702 0.05 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp2 1 Inf 0.025 0.025 0.9 ns
3 Study 1 Inf 24.928 24.928 <0.0001 ***
4 GA_samp 1 Inf 0.504 0.504 0.5 ns
2 Folate_gp2:Study 1 Inf 3.456 3.456 0.06 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp2 1 Inf 1.254 1.254 0.3 ns
3 Study 1 Inf 136.147 136.147 <0.0001 ***
4 GA_samp 1 Inf 0.326 0.326 0.6 ns
2 Folate_gp2:Study 1 Inf 0.255 0.255 0.6 ns

14.5.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 15.45 (10.83, 22.04) <0.0001
Folate_gp2≥40 0.97 (0.92, 1.03) 0.36
StudySTOP 0.99 (0.89, 1.10) 0.86
GA_samp 1.15 (1.13, 1.18) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.84 (1.53, 2.21) <0.0001
Folate_gp2≥40 1.01 (0.98, 1.04) 0.66
StudySTOP 1.12 (1.06, 1.18) 0.0001
GA_samp 1.34 (1.32, 1.36) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.08 (0.06, 0.10) <0.0001
Folate_gp2≥40 0.96 (0.92, 1.00) 0.08
StudySTOP 0.99 (0.92, 1.07) 0.81
GA_samp 1.30 (1.28, 1.32) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 12.48 (7.90, 19.71) <0.0001
Folate_gp2≥40 0.97 (0.89, 1.05) 0.46
StudySTOP 0.80 (0.69, 0.92) 0.002
GA_samp 1.01 (0.99, 1.04) 0.32
HOMA-IR
Var Effect P
(Intercept) 3.16 (1.95, 5.12) <0.0001
Folate_gp2≥40 0.98 (0.90, 1.07) 0.61
StudySTOP 0.69 (0.59, 0.80) <0.0001
GA_samp 1.01 (0.98, 1.04) 0.47
Glucose (mmol/L)
Var Effect P
(Intercept) 5.60 (5.14, 6.11) <0.0001
Folate_gp2≥40 1.01 (0.99, 1.03) 0.22
StudySTOP 0.86 (0.84, 0.88) <0.0001
GA_samp 1.00 (0.99, 1.00) 0.55

14.5.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 97.59156 7.250616 83.77818 84.187 113.1304
STOP 96.70558 6.867894 70.04977 83.934 111.4205
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 88.46211 2.325528 144.1406 83.98294 93.18017
STOP 98.82432 2.380021 101.7462 94.21441 103.65980
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.328197 0.0639117 393.6499 2.205877 2.45730
STOP 2.307065 0.0527727 220.1818 2.205370 2.41345
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 14.94055 0.7244048 Inf 13.58612 16.43001
STOP 11.89215 0.4304671 Inf 11.07768 12.76650
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.632878 0.1870699 Inf 3.284123 4.018669
STOP 2.489792 0.0951203 Inf 2.310168 2.683381
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.500541 0.0501650 Inf 5.403093 5.599747
STOP 4.730796 0.0344955 Inf 4.663667 4.798892

14.5.4 Comparisons (Overall)

Hormone Contrast Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) ≥40 / <40 0.97 (0.92, 1.03) 0.4
hPL (μg/ml) ≥40 / <40 1.01 (0.98, 1.04) 0.7
GH2 (ng/ml) ≥40 / <40 0.96 (0.92, 1.00) 0.08
Insulin (uU/ml) ≥40 / <40 0.97 (0.89, 1.05) 0.5
HOMA-IR ≥40 / <40 0.98 (0.90, 1.07) 0.6
Glucose (mmol/L) ≥40 / <40 1.01 (0.99, 1.03) 0.2

14.5.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.5.6 Plots