14.5 Folate (categories 2)

Folate range (<40 vs >=40)

14.5.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	229.3346032	1	<0.0001	***
Folate_gp2	2.4595598	1	0.1	ns
Study	0.5016894	1	0.5	ns
GA_samp	173.6025363	1	<0.0001	***
Folate_gp2:Study	1.6178809	1	0.2	ns

hPL (μg/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	41.6985648	1	<0.0001	***
Folate_gp2	0.3767370	1	0.5	ns
Study	13.4402945	1	0.0002	***
GA_samp	2493.7981827	1	<0.0001	***
Folate_gp2:Study	0.1843189	1	0.7	ns

GH2 (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	416.3034217	1	<0.0001	***
Folate_gp2	0.5199859	1	0.5	ns
Study	0.0000572	1	1.0	ns
GA_samp	1063.6192459	1	<0.0001	***
Folate_gp2:Study	0.2606543	1	0.6	ns

Insulin (uU/ml)

	model term	df1	df2	F.ratio	Chisq	p.value
1	Folate_gp2	1	Inf	0.141	0.141	0.7	ns
3	Study	1	Inf	10.517	10.517	0.001	**
4	GA_samp	1	Inf	0.967	0.967	0.3	ns
2	Folate_gp2:Study	1	Inf	3.702	3.702	0.05	ns

HOMA-IR

	model term	df1	df2	F.ratio	Chisq	p.value
1	Folate_gp2	1	Inf	0.025	0.025	0.9	ns
3	Study	1	Inf	24.928	24.928	<0.0001	***
4	GA_samp	1	Inf	0.504	0.504	0.5	ns
2	Folate_gp2:Study	1	Inf	3.456	3.456	0.06	ns

Glucose (mmol/L)

	model term	df1	df2	F.ratio	Chisq	p.value
1	Folate_gp2	1	Inf	1.254	1.254	0.3	ns
3	Study	1	Inf	136.147	136.147	<0.0001	***
4	GA_samp	1	Inf	0.326	0.326	0.6	ns
2	Folate_gp2:Study	1	Inf	0.255	0.255	0.6	ns

14.5.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)

Var	Effect	P
(Intercept)	15.45 (10.83, 22.04)	<0.0001
Folate_gp2≥40	0.97 (0.92, 1.03)	0.36
StudySTOP	0.99 (0.89, 1.10)	0.86
GA_samp	1.15 (1.13, 1.18)	<0.0001

hPL (μg/ml)

Var	Effect	P
(Intercept)	1.84 (1.53, 2.21)	<0.0001
Folate_gp2≥40	1.01 (0.98, 1.04)	0.66
StudySTOP	1.12 (1.06, 1.18)	0.0001
GA_samp	1.34 (1.32, 1.36)	<0.0001

GH2 (ng/ml)

Var	Effect	P
(Intercept)	0.08 (0.06, 0.10)	<0.0001
Folate_gp2≥40	0.96 (0.92, 1.00)	0.08
StudySTOP	0.99 (0.92, 1.07)	0.81
GA_samp	1.30 (1.28, 1.32)	<0.0001

Insulin (uU/ml)

Var	Effect	P
(Intercept)	12.48 (7.90, 19.71)	<0.0001
Folate_gp2≥40	0.97 (0.89, 1.05)	0.46
StudySTOP	0.80 (0.69, 0.92)	0.002
GA_samp	1.01 (0.99, 1.04)	0.32

HOMA-IR

Var	Effect	P
(Intercept)	3.16 (1.95, 5.12)	<0.0001
Folate_gp2≥40	0.98 (0.90, 1.07)	0.61
StudySTOP	0.69 (0.59, 0.80)	<0.0001
GA_samp	1.01 (0.98, 1.04)	0.47

Glucose (mmol/L)

Var	Effect	P
(Intercept)	5.60 (5.14, 6.11)	<0.0001
Folate_gp2≥40	1.01 (0.99, 1.03)	0.22
StudySTOP	0.86 (0.84, 0.88)	<0.0001
GA_samp	1.00 (0.99, 1.00)	0.55

14.5.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

PRL (ng/ml)

Study	response	SE	df	lower.CL	upper.CL
SCOPE	97.59156	7.250616	83.77818	84.187	113.1304
STOP	96.70558	6.867894	70.04977	83.934	111.4205

hPL (μg/ml)

Study	response	SE	df	lower.CL	upper.CL
SCOPE	88.46211	2.325528	144.1406	83.98294	93.18017
STOP	98.82432	2.380021	101.7462	94.21441	103.65980

GH2 (ng/ml)

Study	response	SE	df	lower.CL	upper.CL
SCOPE	2.328197	0.0639117	393.6499	2.205877	2.45730
STOP	2.307065	0.0527727	220.1818	2.205370	2.41345

Insulin (uU/ml)

Study	response	SE	df	asymp.LCL	asymp.UCL
SCOPE	14.94055	0.7244048	Inf	13.58612	16.43001
STOP	11.89215	0.4304671	Inf	11.07768	12.76650

HOMA-IR

Study	response	SE	df	asymp.LCL	asymp.UCL
SCOPE	3.632878	0.1870699	Inf	3.284123	4.018669
STOP	2.489792	0.0951203	Inf	2.310168	2.683381

Glucose (mmol/L)

Study	response	SE	df	asymp.LCL	asymp.UCL
SCOPE	5.500541	0.0501650	Inf	5.403093	5.599747
STOP	4.730796	0.0344955	Inf	4.663667	4.798892

14.5.4 Comparisons (Overall)

Hormone	Contrast	Ratio of Geometric means (95% CI)	P-value
PRL (ng/ml)	≥40 / <40	0.97 (0.92, 1.03)	0.4
hPL (μg/ml)	≥40 / <40	1.01 (0.98, 1.04)	0.7
GH2 (ng/ml)	≥40 / <40	0.96 (0.92, 1.00)	0.08
Insulin (uU/ml)	≥40 / <40	0.97 (0.89, 1.05)	0.5
HOMA-IR	≥40 / <40	0.98 (0.90, 1.07)	0.6
Glucose (mmol/L)	≥40 / <40	1.01 (0.99, 1.03)	0.2

14.5.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.5.6 Plots